GMOsHealth & DiseaseSoil Erosion & ContaminationWater Contaminaton & Loss

Widespread Glyphosate Contamination in USA

Most comprehensive study reveals glyphosate and AMPA in the environment over 9 years and across 38 states.

by Dr Eva Sirinathsinghji

The most comprehensive research to date on environmental glyphosate levels exposes the widespread contamination of soil and water in the US, as well as its water treatment system. Looking at a wide range of geographical locations, researchers from the US Geological Survey (USGS) analysed 3732 water and sediment samples and 1081 quality assurance samples collected between 2001 and 2010 from 38 states in the US and the district of Colombia. They found glyphosate in 39.4 % of samples (1470 out of 3732) and its metabolite AMPA (α-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid) in 55% of samples [1]. Water samples included streams, groundwater, ditches and drains, large rivers, soil water, lakes, ponds and wetlands, precipitation, soil and sediment, and waste water treatment plants.

Results expected

These results are to be expected when the use of glyphosate has steadily increased in the US (and similarly in Canada) over the years, particularly since the introduction of genetically-modified crops tolerant to the herbicide. The rise of glyphosate-resistant weeds also means that farmers need to spray more chemicals than before in order to protect their crops (see [2] Monsanto Defeated by Roundup Resistant Weeds, SiS 53). Glyphosate accounted for 32-36% of all pesticide (insecticides, herbicides and fungicides) use in the US in 2007 according to EPA data [3]. It is the top pesticide in agriculture and the second for home and garden and commercial settings. Agricultural use has gone up from 3 180 tonnes (of active ingredient) in 1987 to 82800 tonnes in 2007. Non-agricultural use of the herbicide has also risen steadily in the US, from 2270 tonnes in 1993 to 9300 tonnes in 2007 (Figure 1). The common use of glyphosate in urban areas is also exacerbated by the impervious surfaces of cities, resulting in substantial pesticide inputs to urban drainage systems. Until recently data had been lacking on glyphosate occurrence in the environment, though studies published over the last couple of years are raising concerns. Detecting glyphosate in surface waters, rain and even groundwater, contradicts the producers’ claim that its chemical propensity to bind to sediment will prevent it from leaching into groundwater supplies (see [4] GM Crops and Water – A Recipe for Disaster, SiS 58).

Figure 1: Use of glyphosate and planted hectares of corn and soybeans from 1987-2008

Data collection had previously been limited not only by glyphosate’s high solubility and polarity which make its detection more difficult, especially at environmentally relevant levels, but also due to the official line taken by authorities that glyphosate is safe. This makes assessment of its presence in our environment less of a priority, and hence left unstudied and unregulated. The safety claim has also encouraged farmers to overuse glyphosate, mostly sprayed on crops “post-emergence” or after crops and weeds have emerged from the soil and often applied repeatedly throughout the season, especially with the rise of glyphosate-resistant weeds. In addition, they are liberally used on non-GM crops as a dessicant (drying agent) to facilitate harvesting (see [5] How Roundup® Poisoned my Nature Reserve, SiS 64).

To address the lack of knowledge in this area, researchers at the USGS began developing their own methods in the 2000s, using solid-phase extraction and liquid chromatography/mass spectroscopy, which is able to detect both glyphosate and its metabolite AMPA at levels as low as 0.02 μg/l (0.02 part per billion, ppb) for both compounds.

The results are shown in Table 1. Glyphosate and AMPA were most frequently detected in soil, followed by drains and ditches, rain and large rivers. For soil and sediment, and soil water a total of 45 soil and sediment samples were collected from seven sites in Mississippi and Indiana, with both glyphosate and AMPA being detected at least once in samples from all seven sites. Both were detected in 90 % of sediment samples with concentrations frequently above 10 μg/kg, with an average of 9.6 μg/kg. In 116 soil samples glyphosate and AMPA were detected in 34.5 % and 66.5 % respectively. Large rivers showed average levels of 0.03 μg/kg in 53.1 % of samples tested. Least frequent but detectable levels were found in groundwater samples, with 5.8 % and 14 % of samples testing positive for glyphosate and AMPA respectively.

Glyphosate is claimed by biotech proponents not to leach into groundwater supplies, but this work and a previous study performed in Catalonia, Spain have both detected its presence in groundwater supplies [4], a major source of drinking water.

The present study also found an increase in concentrations over time, showing higher levels from 2006-2010 compared to earlier years (2001-2005), consistent with rises in both agricultural, home and commercial use of the herbicide. Temporal patterns however, were not recorded and these likely change with agricultural seasons.

The study highlights the ubiquitous contamination of the environment with glyphosate herbicides at ever increasing levels. This herbicide is highly toxic to humans, farm animals, and wildlife, and at levels as low as 0.1 ppb; there is indeed a strong case for halting its use altogether (see [6] Ban GMOs Now, Special ISIS report).

Hydrologic Setting

Number of Samples

Percentage and (number) with Glyphosate Detections

Median Glyphosate in μg/l or μg/kg

Maximum Glyphosate in μg/l or μg/kg

Percentage and (number) with AMPA Detections

Median AMPA in μg/l or μg/kg

Maximum AMPA in μg/l or μg/kg

All sites

3 732

39.4 (1,470)



55.0 (2,052)




1 508

52.5 (791)



71.6 (1,079)




1 171

5.8 (68)



14.3 (168)



Ditches and drains


70.9 (265)



80.7 (302)



Large rivers


53.1 (169)



89.3 (284)



Soil water


34.5 (40)



65.5 (76)



Lakes, ponds, and wetlands


33.7 (35)



29.8 (31)





70.6 (60)



71.8 (61)



Soil and sediment


91.1 (41)



93.3 (42)



WWTP outfall


9.09 (1)



81.8 (9)



Table 1: Concentrations of both glyphosate and its metabolite AMPA in US environment


  1. Battaglin WA, Meyer MT, Kuivila KM, and Dietze JE. Glyphosate and Its Degradation Product AMPA Occur Frequently and Widely in U.S. Soils, Surface Water, Groundwater, and Precipitation. Journal of the American Water Resources Association (JAWRA) 2014, 50, 275-290. DOI: 10.1111/jawr.12159
  2. Sirinathsinghji E. Monsanto Defeated By Roundup Resistant Weeds. Science in Society 53, 40-41, 2011.
  3. 2006-2007 Pesticide Market Estimates, 3.4 Amount of Pesticides Used in the United States: Conventional. US Environmental Protection Agency.
  4. Sirinathsinghji E. GM Crops and Water – A Recipe for Disaster. Science in Society 58, 8-10, 2013.
  5. Mason, R. How Roundup Poisoned My Nature Reserve, SiS 64, to appear
  6. Ho MW and Sirinathsinghji E. Ban GMOs Now, ISIS, London, June 2013,


  1. Chris Grue of the U of Washington –his abstract 2003 found glyphosate in the gonads of
    Willapa Bay oysters. Willapa Bay had been sprayed for at least 15 years with glyphosate, and subsequently with a cocktail–addition of imazapyr. A public info request was filed and alot of information re the abstract was received, however the study on which it was based was not released and attempts to find it have been unsuccessful. If anyone has any information on this, or contamination of oysters in Willapa Bay please let me know.

  2. I’m curious what the author thinks of the fact that glyphosate is less toxic than caffeine and chocolate. What researching is being used to claim that it is highly toxic to life? Any research I’ve come across points to the opposite, even including soil organisms, which it apparently stimulates rather than harms.

    1. You can’t be serious Robert?! As someone who’s covered this issue extensively for over 10 years, the damning evidence is overwhelming. I’d be interested to see what research you’ve “come across”, as well who the authors were.

      I’m glad to see the chickens are finally coming home to roost on this issue. While I’m not a fan of lawsuits as a solution to our worldly problems, this is the only way that Bayer/Monsanto is ever going to begin to take responsibility for their actions.

      Here’s one of our latest articles where we talk about this and the impact it has on our water:

  3. Glyphosate is a chemical that can be found in the weed killer which is popular around the world. It was discovered in the 1950s, and the Monsanto company has patented it. They have included it in the ‘RoundUp’ product, and since then, many people have been using it in their plants.

    The chemical name of glyphosate is ‘N-(phosphonomethyl)glycine.’ It has been used in over 160 countries worldwide with over 650,000 tons in 2011. It is primarily used in agriculture and big farming, however, you can use it in home gardening and other public places.

    Further reading:

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